ID   pGB-10-11-ccdB; SV 1; circular; unknown; STD; UNC; 3470 BP.
AC   unknown;
DT   02-DEC.-2020
DE   LMBP 12034.
OS   synthetic DNA construct
OC   synthetic DNA construct
CC   Accession number: LMBP 12034
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 2-DEC-2020
FH   Key             Location/Qualifiers
FH
FT   misc_feature    1..224
FT                   /label="Tn7 recombination sequence"
FT   CDS             complement(291..824)
FT                   /label="gentamycin resistance"
FT   promoter        891..925
FT                   /label="intl1 promoter"
FT   promoter        complement(1013..1041)
FT                   /label="PcS promoter"
FT   misc_feature    1112..1145
FT                   /label="loxP"
FT   misc_feature    1183..1186
FT                   /label="GoldenBac overhang 10"
FT   promoter        1237..1325
FT                   /label="AcNPV-PH promoter"
FT   misc_feature    1396..1479
FT                   /label="Twin-Strep-tag"
FT   misc_feature    1489..1512
FT                   /label="PreScission site"
FT   CDS             1638..1946
FT                   /label="ccdB"
FT   misc_feature    1966..1983
FT                   /label="His-tag"
FT   terminator      complement(2105..2201)
FT                   /label="SV40 polyA late"
FT   terminator      2105..2201
FT                   /label="SV40 polyA early"
FT   misc_feature    2382..2385
FT                   /label="GoldenBac overhang 11"
FT   misc_feature    2393..2558
FT                   /label="Tn7 recombination sequence"
FT   rep_origin      3118..3421
FT                   /label="pMB1 ori"
SQ   SEQUENCE  3470 AA; 1071713 MW;
     TGTGGGCGGA CAATAAAGTC TTAAACTGAA CAAAATAGAT CTAAACTATG ACAATAAAGT        60
     CTTAAACTAG ACAGAATAGT TGTAAACTGA AATCAGTCCA GTTATGCTGT GAAAAAGCAT       120
     ACTGGACTTT TGTTATGGCT AAAGCAAACT CTTCATTTTC TGAAGTGCAA ATTGCCCGTC       180
     GTATTAAAGA GGGGCGTGGC CAAGGGCATG TAAAGACTAT ATTCGCGGCG TTGTGACAAT       240
     TTACCGAACA ACTCCGCGGC CGGGAAGCCG ATCTCGGCTT GAACGAATTG TTAGGTGGCG       300
     GTACTTGGGT CGATATCAAA GTGCATCACT TCTTCCCGTA TGCCCAACTT TGTATAGAGA       360
     GCCACTGCGG GATCGTCACC GTAATCTGCT TGCACGTAGA TCACATAAGC ACCAAGCGCG       420
     TTGGCCTCAT GCTTGAGGAG ATTGATGAGC GCGGTGGCAA TGCCCTGCCT CCGGTGCTCG       480
     CCGGAGACTG CGAGATCATA GATATAGATC TCACTACGCG GCTGCTCAAA CTTGGGCAGA       540
     ACGTAAGCCG CGAGAGCGCC AACAACCGCT TCTTGGTCGA AGGCAGCAAG CGCGATGAAT       600
     GTCTTACTAC GGAGCAAGTT CCCGAGGTAA TCGGAGTCCG GCTGATGTTG GGAGTAGGTG       660
     GCTACGTCTC CGAACTCACG ACCGAAAAGA TCAAGAGCAG CCCGCATGGA TTTGACTTGG       720
     TCAGGGCCGA GCCTACATGT GCGAATGATG CCCATACTTG AGCCACCTAA CTTTGTTTTA       780
     GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTGCGTA ACATCGTTGC TGCTCCATAA       840
     CATCAAACAT CGACCCACGG CGTAACGCGC TTGCTGCTTG GATGCCCGAG GCATAGACTG       900
     TACAAAAAAA CAGTCATAAC AAGCCATGAA AACCGCCACT GCGCCGTTAC CACCGCTGCG       960
     TTCGGTCAAG GTTCTGGACC AGTTGCGTGA GCGCATACGC TACTTGCATT ACAGTTTACG      1020
     AACCGAACAG GCTTATGTCA ACTGGGTTCG TGCCTTCATC CGTTTCCACG GTGTGCGTCA      1080
     CCCGGCAACC TTGGGCAGCA GCGAAGTCGC CATAACTTCG TATAGCATAC ATTATACGAA      1140
     GTTATCTGTA ACTATAACGG TCCTAAGGTA GCGAAGGTCT CAAAGCGTTT AAACACTAGT      1200
     ATCGATTCGC GACCTACTCC GGAATATTAA TAGATCATGG AGATAATTAA AATGATAACC      1260
     ATCTCGCAAA TAAATAAGTA TTTTACTGTT TTCGTAACAG TTTTGTAATA AAAAAACCTA      1320
     TAAATATTCC GGATTATTCA TACCGTCCCA CCATCGGGCG ACGGATCCCG GTCCGAAACC      1380
     ATGAAAGGGA GCGCTTGGAG CCACCCGCAG TTCGAAAAAG GTGGAGGTTC TGGCGGTGGA      1440
     TCGGGAGGTT CAGCGTGGAG CCACCCGCAG TTCGAGAAAA CCGCGGGTCT GGAAGTTCTG      1500
     TTCCAGGGGC CCATGGACTA ATGAGGTACC GGATCCGAAT TCGAGCTCCG TCGACAAGCT      1560
     TGATATCGAA TTCCTGCAGC CCAAACTGCA GTTGACAACA TAAAAACTTT GTGTTATACT      1620
     TGTAACGTAA GGAGGTAATG ATTCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT      1680
     ATCGTCTGTT TGTGGATGTA CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA      1740
     TCCCCCTGGC CAGTGCACGT CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG      1800
     TGCATATCGG GGATGAAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT      1860
     CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA      1920
     TTAACCTGAT GTTCTGGGGA ATATAAGCTT GCGGCCGCAC TCGAGCACCA CCACCACCAC      1980
     CACTGACATG CGGTACCAAG CTTGTCGAGA AGTACTAGAG GATCATAATC AGCCATACCA      2040
     CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC      2100
     ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT      2160
     AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG      2220
     GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CTGATCACTG CTTGAGCCTA      2280
     GAAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG      2340
     CAATAACTAT CATAACCCCT AGGGTATACC CATCTAATTG GAGAATGAGA CCAACCAGAT      2400
     AAGTGAAATC TAGTTCCAAA CTATTTTGTC ATTTTTAATT TTCGTATTAG CTTACGACGC      2460
     TACACCCAGT TCCCATCTAT TTTGTCACTC TTCCCTAAAT AATCCTTAAA AACTCCATTT      2520
     CCACCCCTCC CAGTTCCCAA CTATTTTGTC CGCCCACAAC CGGTTGACTT GGGTCAACTG      2580
     TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA      2640
     AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT      2700
     TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT      2760
     TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT      2820
     TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG      2880
     ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA      2940
     GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT      3000
     AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG      3060
     GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG      3120
     AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC      3180
     AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA      3240
     AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT      3300
     TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA      3360
     CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT      3420
     TGACTTGGGT CGCTCTTCCT GTGGATGCGC AGATGCCCTG CGTAAGCGGG                 3470
//
