ID   pGBdest_ccdB; SV 1; circular; unknown; STD; UNC; 4958 BP.
AC   unknown;
DT   30-JUL.-2020
DE   LMBP 12024.
OS   synthetic DNA construct
OC   synthetic DNA construct
CC   Accession number: LMBP 12024
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 30-JUL-2020
FH   Key             Location/Qualifiers
FH
FT   CDS             complement(1..534)
FT                   /label="gentamycin resistance"
FT   promoter        601..635
FT                   /label="intl1 promoter"
FT   promoter        complement(723..751)
FT                   /label="PcS promoter"
FT   misc_feature    860..863
FT                   /label="GoldenBac overhang 01"
FT   CDS             complement(1184..1489)
FT                   /label="ccdB"
FT   CDS             complement(1508..2167)
FT                   /label="chloramphenicol resistance"
FT   misc_feature    2305..2308
FT                   /label="overhang 16"
FT   misc_feature    2317..2482
FT                   /label="Tn7 recombination sequence"
FT   CDS             complement(2838..3653)
FT                   /label="Tn903 neomycin resistance"
FT   rep_origin      4145..4547
FT                   /label="pMB1 ori"
FT   misc_feature    4668..4892
FT                   /label="Tn7 recombination sequence"
SQ   SEQUENCE  4958 AA; 1530117 MW;
     TTAGGTGGCG GTACTTGGGT CGATATCAAA GTGCATCACT TCTTCCCGTA TGCCCAACTT        60
     TGTATAGAGA GCCACTGCGG GATCGTCACC GTAATCTGCT TGCACGTAGA TCACATAAGC       120
     ACCAAGCGCG TTGGCCTCAT GCTTGAGGAG ATTGATGAGC GCGGTGGCAA TGCCCTGCCT       180
     CCGGTGCTCG CCGGAGACTG CGAGATCATA GATATAGATC TCACTACGCG GCTGCTCAAA       240
     CTTGGGCAGA ACGTAAGCCG CGAGAGCGCC AACAACCGCT TCTTGGTCGA AGGCAGCAAG       300
     CGCGATGAAT GTCTTACTAC GGAGCAAGTT CCCGAGGTAA TCGGAGTCCG GCTGATGTTG       360
     GGAGTAGGTG GCTACGTCTC CGAACTCACG ACCGAAAAGA TCAAGAGCAG CCCGCATGGA       420
     TTTGACTTGG TCAGGGCCGA GCCTACATGT GCGAATGATG CCCATACTTG AGCCACCTAA       480
     CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTGCGTA ACATCGTTGC       540
     TGCTCCATAA CATCAAACAT CGACCCACGG CGTAACGCGC TTGCTGCTTG GATGCCCGAG       600
     GCATAGACTG TACAAAAAAA CAGTCATAAC AAGCCATGAA AACCGCCACT GCGCCGTTAC       660
     CACCGCTGCG TTCGGTCAAG GTTCTGGACC AGTTGCGTGA GCGCATACGC TACTTGCATT       720
     ACAGTTTACG AACCGAACAG GCTTATGTCA ACTGGGTTCG TGCCTTCATC CGTTTCCACG       780
     GTGTGCGTCA CCCGGCAACC TTGGGCAGCA GCGAAGTCGA GGCATTTCTG TCCTGGCTGG       840
     CGAACGAGCG CAAGGTTTCA CCTTGAGACC GAATTCAAAA AAAAGCGGAC CTCCGAAGAG       900
     GTCCGCCAGA TGATCATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT       960
     GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA      1020
     GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG      1080
     CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC      1140
     GACGTTGTAA AACGACGGCC AGCATTGATT ACCTCCTACT AGTTTATATT CCCCAGAACA      1200
     TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC ACTTCTTCCC      1260
     CGATAACGGA GACTGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG CTTTCATCCC      1320
     CGATATGCAC CACTGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA CGTGCACTGG      1380
     CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT ACATCCACAA      1440
     ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATA GCTGTCCTCC      1500
     TGAGCTCTTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC      1560
     TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA      1620
     CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA      1680
     TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA      1740
     ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT      1800
     CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG      1860
     AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA      1920
     CCAGCTCACC GTCTTTCATT GCCATACGGA ACTCCGGATG AGCATTCATC AGGCGGGCAA      1980
     GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG      2040
     CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT      2100
     CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT      2160
     TCTCCATAGC TGACCTCCTA AGCTTAGCAC ACCTCTTTTT GACATACTTC GGGTATACAT      2220
     ATCAGTATAT ATTCTTATAC CGCAAAAATC AGCGCGCAAA TACGCATACT GTTATCTGGC      2280
     TTTTAGTAAG CCTCGAGGGT CTCAGTATGT TTAAACAACC AGATAAGTGA AATCTAGTTC      2340
     CAAACTATTT TGTCATTTTT AATTTTCGTA TTAGCTTACG ACGCTACACC CAGTTCCCAT      2400
     CTATTTTGTC ACTCTTCCCT AAATAATCCT TAAAAACTCC ATTTCCACCC CTCCCAGTTC      2460
     CCAACTATTT TGTCCGCCCA CAGCGGGGCA TTTTTCTTCC TGTTATGTTT TTAATCAAAC      2520
     ATCCTGCCAA CTCCATGTGA CAAACCGTCA TCTTCGGCTA CTTTTTCTCT GTCACAGAAT      2580
     GAAAATTTTT CTGTCATCTC TTCGTTATTA ATGTTTGTAA TTGACTGAAT ATCAACGCTT      2640
     ATTTGCAGCC TGAATGGCGA ATGGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT      2700
     GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC      2760
     GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCAAACGTA CTGCGGTAAA GCTCATCAGC      2820
     GTGGTCGTGA AGAATTCTTA GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC      2880
     ATATCAGGAT TATCAATACC ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC      2940
     TCACCGAGGC AGTTCCATAG GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT      3000
     CCAACATCAA TACAACCTAT TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA      3060
     TCACCATGAG TGACGACTGA ATCCGGTGAG AATGGCAAAA GTTTATGCAT TTCTTTCCAG      3120
     ACTTGTTCAA CAGGCCAGCC ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG      3180
     TTATTCATTC GTGATTGCGC CTGAGCGAGA CGAAATACGC GATCGCTGTT AAAAGGACAA      3240
     TTACAAACAG GAATCGAATG CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT      3300
     TCACCTGAAT CAGGATATTC TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG      3360
     GTGAGTAACC ATGCATCATC AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA      3420
     AATTCCGTCA GCCAGTTTAG TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT      3480
     TTGCCATGTT TCAGAAACAA CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC      3540
     GCACCTGATT GCCCGACATT ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG      3600
     TTGGAATTTA ATCGCGGCCT AGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC      3660
     CTTGTATTAC TGTTTATGTA AGCAGACAGT TTTATTGTTC ATGACCAAAA TCCCTTAACG      3720
     TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA      3780
     TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT      3840
     GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG      3900
     AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA      3960
     CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG      4020
     TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA      4080
     GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC      4140
     CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA      4200
     GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC      4260
     AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG      4320
     TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC      4380
     CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC      4440
     CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG      4500
     CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA      4560
     TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA GACCAGCCGC GTAACCTGGC      4620
     AAAATCGGTT ACGGTTGAGT AATAAATGGA TGCCCTGCGT AAGCGGGTGT GGGCGGACAA      4680
     TAAAGTCTTA AACTGAACAA AATAGATCTA AACTATGACA ATAAAGTCTT AAACTAGACA      4740
     GAATAGTTGT AAACTGAAAT CAGTCCAGTT ATGCTGTGAA AAAGCATACT GGACTTTTGT      4800
     TATGGCTAAA GCAAACTCTT CATTTTCTGA AGTGCAAATT GCCCGTCGTA TTAAAGAGGG      4860
     GCGTGGCCAA GGGCATGGTA AAGACTATAT TCGCGGCGTT GTGACAATTT ACCGAACAAC      4920
     TCCGCGGCCG GGAAGCCGAT CTCGGCTTGA ACGAATTG                              4958
//
